Overview

  • Product nameAnti-RPP40 antibody
    See all RPP40 primary antibodies
  • Description
    Mouse polyclonal to RPP40
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-244 of Human RPP40 (AAH17871.1)

  • Positive control
    • human kidney lysate, in transfected 293T cell line, HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab89264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.
    • Cellular localizationNucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 3.1.26.5 antibody
      • Ribonuclease P protein subunit p40 antibody
      • RNase P subunit 1 antibody
      • RNaseP protein p40 antibody
      • RNASEP1 antibody
      • RPP40 antibody
      • RPP40_HUMAN antibody
      see all

    Anti-RPP40 antibody images

    • Anti-RPP40 antibody (ab89264) at 5 µg/ml + human kidney lysate at 50 µg

      Secondary
      HRP goat anti mouse IgG at 2500

      Predicted band size : 35 kDa
    • All lanes : Anti-RPP40 antibody (ab89264) at 5 µg/ml

      Lane 1 : transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 35 kDa
    • Immunofluorescent analysis of RPP40 expression in HeLa cells using ab89264 at 10 ug/ml

    References for Anti-RPP40 antibody (ab89264)

    ab89264 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab89264.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"