Recombinant
RabMAb

Anti-RPS15 antibody [EPR11104] (ab157193)

Overview

  • Product name
    Anti-RPS15 antibody [EPR11104]
    See all RPS15 primary antibodies
  • Description
    Rabbit monoclonal [EPR11104] to RPS15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).

  • Positive control
    • A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11104
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U-87 MG cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.
    • Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
    • Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.

    References

    ab157193 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157193.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up