Overview

  • Product name
  • Description
    Rabbit polyclonal to RPS19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Fish, Rhesus monkey, Gorilla, Orangutan, Frog
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 95-145 of Human RPS19 (NP_001013.1).

  • Positive control
    • HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab168840 was affinity purified using an epitope specific to RPS19 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 16 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
  • Tissue specificity
    Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
  • Involvement in disease
    Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein S19e family.
  • Cellular localization
    Nucleus. Located more specifically in the nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S19 antibody
    • DBA antibody
    • DBA1 antibody
    • HGNC:10402 antibody
    • Ribosomal protein S19 antibody
    • rps19 antibody
    • RS19_HUMAN antibody
    • S19 antibody
    see all

Images

  • All lanes : Anti-RPS19 antibody (ab168840) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa


    Exposure time: 3 minutes
  • Detection of RPS19 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168840 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab168840 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab168840.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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