Overview

  • Product name
  • Description
    Goat polyclonal to RPS19
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide: with C-

    KMVEKDQDGGRK

    sequence from the internal region of Human RPS19.

  • Positive control
    • Daudi and K562 cell lysates. Lysate prepared from transfected HEK293 transiently expressing RPS19.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20206555
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).

Target

  • Function
    Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
  • Tissue specificity
    Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
  • Involvement in disease
    Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein S19e family.
  • Cellular localization
    Nucleus. Located more specifically in the nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S19 antibody
    • DBA antibody
    • DBA1 antibody
    • HGNC:10402 antibody
    • Ribosomal protein S19 antibody
    • rps19 antibody
    • RS19_HUMAN antibody
    • S19 antibody
    see all

Images

References

This product has been referenced in:
  • Bibikova E  et al. TNF-mediated inflammation represses GATA1 and activates p38 MAP kinase in RPS19-deficient hematopoietic progenitors. Blood 124:3791-8 (2014). Read more (PubMed: 25270909) »
  • Horos R  et al. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood 119:262-72 (2012). WB ; Mouse . Read more (PubMed: 22058113) »

See all 3 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Sorted bone marrow cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient Bis-Tris gel)
Loading amount
50000 cells
Specification
Sorted bone marrow cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Le Qi

Verified customer

Submitted Feb 21 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Goat Cell lysate - whole cell (keratinocytes)
Loading amount
30 µg
Specification
keratinocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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