• Product nameAnti-RPS19 antibody
    See all RPS19 primary antibodies
  • Description
    Mouse monoclonal to RPS19
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-146 of Human RPS19


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab57643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • FunctionRequired for pre-rRNA processing and maturation of 40S ribosomal subunits.
  • Tissue specificityHigher level expression is seen in the colon carcinoma tissue than normal colon tissue.
  • Involvement in diseaseDefects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similaritiesBelongs to the ribosomal protein S19e family.
  • Cellular localizationNucleus. Located more specifically in the nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S19 antibody
    • DBA antibody
    • DBA1 antibody
    • HGNC:10402 antibody
    • Ribosomal protein S19 antibody
    • rps19 antibody
    • RS19_HUMAN antibody
    • S19 antibody
    see all

Anti-RPS19 antibody images

  • Predicted band size : 16 kDa
    RPS19 antibody (ab57643) at 1ug/lane + K-562 cell lysate at 25ug/lane.
  • IHC-P image of RPS19 antibody (ab57643) on Kidney sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/100 dilution for 2 hours.

    See Abreview

  • Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-RPS19 antibody (ab57643)

This product has been referenced in:
  • Knight JR  et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res 41:4185-97 (2013). WB ; Human . Read more (PubMed: 23462953) »
  • Shah ZH  et al. A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating tumor suppressor p53 and governs expression of cancer-related genes. Mol Cell Biol 32:704-16 (2012). WB ; Human . Read more (PubMed: 22124156) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C

Dr. Aamir Ahmed

Verified customer

Submitted Oct 05 2011