The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in disease
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Belongs to the ribosomal protein S19e family.
Nucleus. Located more specifically in the nucleoli.
Predicted band size : 16 kDa RPS19 antibody (ab57643) at 1ug/lane + K-562 cell lysate at 25ug/lane.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS19 antibody (ab57643)This image is courtesy of an abreview submitted by Aamir Ahmed, University College London, United Kingdom.
IHC-P image of RPS19 antibody (ab57643) on Kidney sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/100 dilution for 2 hours.
Overlay histogram showing HCT116 cells stained with ab57643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57643, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Knight JR et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res41:4185-97 (2013).
Read more (PubMed: 23462953) »
Shah ZH et al. A deacetylase-deficient SIRT1 variant opposes full-length SIRT1 in regulating tumor suppressor p53 and governs expression of cancer-related genes. Mol Cell Biol32:704-16 (2012).
Read more (PubMed: 22124156) »