Recombinant
RabMAb

Anti-RPS19 antibody [EPR10423] (ab181365)

Overview

  • Product name
    Anti-RPS19 antibody [EPR10423]
    See all RPS19 primary antibodies
  • Description
    Rabbit monoclonal [EPR10423] to RPS19
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS19BP1 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P39019

  • Positive control
    • K562, 293T, Caco-2 and HeLa cell lysates; Human colonic adenocarcinoma and testis tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10423
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
    • Tissue specificity
      Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
    • Involvement in disease
      Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
    • Sequence similarities
      Belongs to the ribosomal protein S19e family.
    • Cellular localization
      Nucleus. Located more specifically in the nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40S ribosomal protein S19 antibody
      • DBA antibody
      • DBA1 antibody
      • HGNC:10402 antibody
      • Ribosomal protein S19 antibody
      • rps19 antibody
      • RS19_HUMAN antibody
      • S19 antibody
      see all

    Images

    • All lanes : Anti-RPS19 antibody [EPR10423] (ab181365) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 16 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RPS19 with ab181365 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling RPS19 with ab181365 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from Caco-2 cell lysate immunoprecipitated using ab181365 at 1/10 dilution.

    References

    ab181365 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (iPSC and iPSC derived myeloid cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Specification
    iPSC and iPSC derived myeloid cell
    Blocking step
    Odyssey blocking buffer as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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