Recombinant Anti-RPS19 antibody [EPR10423] (ab181365)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10423] to RPS19
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RPS19 antibody [EPR10423]
See all RPS19 primary antibodies -
Description
Rabbit monoclonal [EPR10423] to RPS19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, 293T, Caco-2 and HeLa cell lysates; Human colonic adenocarcinoma and testis tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10423 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Required for pre-rRNA processing and maturation of 40S ribosomal subunits. -
Tissue specificity
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue. -
Involvement in disease
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. -
Sequence similarities
Belongs to the ribosomal protein S19e family. -
Cellular localization
Nucleus. Located more specifically in the nucleoli. - Information by UniProt
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Database links
- Entrez Gene: 6223 Human
- Entrez Gene: 100503302 Mouse
- Entrez Gene: 20085 Mouse
- Entrez Gene: 29287 Rat
- Omim: 603474 Human
- SwissProt: P39019 Human
- SwissProt: Q9CZX8 Mouse
- SwissProt: P17074 Rat
see all -
Alternative names
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
see all
Images
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All lanes : Anti-RPS19 antibody [EPR10423] (ab181365) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa -
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RPS19 with ab181365 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling RPS19 with ab181365 at 1/100 dilution.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from Caco-2 cell lysate immunoprecipitated using ab181365 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181365 has not yet been referenced specifically in any publications.