Overview

  • Product name
    Anti-RPS21 antibody
  • Description
    Rabbit polyclonal to RPS21
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide within residues: QNDAGEFVDL YVPRKCSASN RIIGAKDHAS IQMNVAEVDK VTGRFNGQFK, corresponding to N terminal amino acids 2-51 of Human RPS21 (NP_001015).

  • Positive control
    • Human placenta lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S21E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants that encode different protein isoforms have been described, but their existence has not been verified. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • Cellular localization
    cytosolic small ribosomal subunit
  • Database links
  • Alternative names
    • 1810049N11Rik antibody
    • 2410030A14Rik antibody
    • 40S ribosomal protein S21 antibody
    • 8.2 kDa differentiation factor antibody
    • MGC107620 antibody
    • OTTHUMP00000031485 antibody
    • Ribosomal protein S21 antibody
    • RP23-16P3.4 antibody
    • RP5-908M14.8 antibody
    see all

Images

  • Anti-RPS21 antibody (ab90874) at 1 µg/ml + Human placenta lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 9 kDa
  • IHC-P image of RPS21 antibody (ab90874) on Colon sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 minutes at 22°C, before addition of the primary antibody at 1/750 dilution for 2 hours.

    See Abreview

References

This product has been referenced in:
  • Janssens GE & Veenhoff LM The Natural Variation in Lifespans of Single Yeast Cells Is Related to Variation in Cell Size, Ribosomal Protein, and Division Time. PLoS One 11:e0167394 (2016). WB ; Saccharomyces cerevisiae . Read more (PubMed: 27907085) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization
No
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Oct 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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