Overview

  • Product name
  • Description
    Mouse monoclonal to RPS23
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ASHAKGIVLE KVGVEAKQPN SAIRKCVRVQ LIKNGKKITA FVPNDGCLNF IEENDEVLVA GFGRKGHAVG DIPGVRFKVV KVANVSLLAL YKGKKERPRS , corresponding to amino acids 44-144 of Human RPS23

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 10 µg/ml.

Target

Anti-RPS23 antibody images



  • Predicted band size : 16 kDa
    RPS23 antibody (ab57644) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57644 staining RPS23 (right) compared to control IgG (left) in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 2% BSA for 1 hour at 21°C. Samples were incubated with the primary antibody (2.5µg/ml in PBS + 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Counterstained with DAPI.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab57644 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57644, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ICC/IF image of ab57644 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57644, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RPS23 antibody (ab57644)

This product has been referenced in:
  • Sbarrato T  et al. A ribosome-related signature in peripheral blood CLL B cells is linked to reduced survival following treatment. Cell Death Dis 7:e2249 (2016). Read more (PubMed: 27253413) »
  • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.1% Triton X-100
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 18 2017

ab57644, anti-RPS23, code de remise : ******** ab10361, anti-Pumilio 2, code de remise : ******** Date d’expiration pour ces deux codes : JJ MM AAAA. Je suis ravi que vous souhaitiez participer à notre offre et tester ab10361 and ab57644 c...

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Merci de nous avoir contactés. Nous serons ravis de vous envoyer un code de test pour des anticorps anti-RPS23, anti-pumilio2 et anti-RRM2. Avant l'édition de ces codes, je vous invite à consulter les listes des six anti-pumilio2 et des quatre anti-RRM...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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