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Rabbit polyclonal to RPS3
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Corn, Salamander, Chinese hamster, Orangutan, African bush elephant, Spotted catshark
Synthetic peptide corresponding to a region within amino acids 193-243 of Human RPS3 (NP_000996.2).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the ribosomal protein S3P family.
Contains 1 KH type-2 domain.
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Information by UniProt
40S ribosomal protein S3 antibody
Immunoprecipitation - Anti-RPS3 antibody (ab140688)
Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140688 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-RPS3 antibody (ab140688)
All lanes : Anti-RPS3 antibody (ab140688) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 27 kDa Exposure time: 10 seconds
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