Overview

  • Product name
  • Description
    Rabbit polyclonal to RPS3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-243 of Human RPS3 (Uniprot ID: P23396).

  • Positive control
    • Raji, C8D30 and PC-12 whole cell lysates. SAS xenograft tissue. HeLa cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities
    Belongs to the ribosomal protein S3P family.
    Contains 1 KH type-2 domain.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S3 antibody
    • fb13d09 antibody
    • FLJ26283 antibody
    • FLJ27450 antibody
    • IMR 90 ribosomal protein S3 antibody
    • MGC56088 antibody
    • MGC87870 antibody
    • OTTHUMP00000229804 antibody
    • OTTHUMP00000229805 antibody
    • OTTHUMP00000229874 antibody
    • OTTHUMP00000229877 antibody
    • OTTHUMP00000229878 antibody
    • OTTHUMP00000229879 antibody
    • OTTHUMP00000229880 antibody
    • OTTHUMP00000229882 antibody
    • OTTHUMP00000229883 antibody
    • OTTHUMP00000229886 antibody
    • Ribosomal protein S3 antibody
    • rps3 antibody
    • RS3_HUMAN antibody
    • S3 antibody
    • wu:fb13d09 antibody
    • zgc:56088 antibody
    see all

Images

  • Anti-RPS3 antibody (ab154953) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Anti-RPS3 antibody (ab154953) at 1/2000 dilution + PC-12 whole cell
    lysate/extract at 30 µg

    Predicted band size: 27 kDa



    12% SDS-PAGE
  • Anti-RPS3 antibody (ab154953) at 1/2000 dilution + C8D30 whole cell
    lysate/extract at 30 µg

    Predicted band size: 27 kDa



    12% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded Human SAS xenograft tissue labeling RPS3 with ab154953 at 1/500 dilution.
  • Confocal Immunofluorescence analysis of methanol-fixed HeLa cells labeling RPS3 with ab154953 at 1/500 dilution (green). Alpha tubulin filaments are labeled with a mouse monoclonal antibody at 1/2000 dilution (red).

References

ab154953 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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