Overview

  • Product nameAnti-RPS3 antibody
    See all RPS3 primary antibodies
  • Description
    Mouse monoclonal to RPS3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment: GQRAKSMKFV DGLMIHSGDP VNYYVDTAVR HVLLRQGVLG IKVKIMLPWD PTGKIGPKKP LPDHVSIVEP KDEILPTTPI SEQKGGKPEP PAMPQPVPTA , corresponding to amino acids 144-243 of Human RPS3 with a 26kDa tag.

  • Positive control
    • HeLa nuclear extract

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Sequence similaritiesBelongs to the ribosomal protein S3P family.
    Contains 1 KH type-2 domain.
  • Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S3 antibody
    • fb13d09 antibody
    • FLJ26283 antibody
    • FLJ27450 antibody
    • IMR 90 ribosomal protein S3 antibody
    • MGC56088 antibody
    • MGC87870 antibody
    • OTTHUMP00000229804 antibody
    • OTTHUMP00000229805 antibody
    • OTTHUMP00000229874 antibody
    • OTTHUMP00000229877 antibody
    • OTTHUMP00000229878 antibody
    • OTTHUMP00000229879 antibody
    • OTTHUMP00000229880 antibody
    • OTTHUMP00000229882 antibody
    • OTTHUMP00000229883 antibody
    • OTTHUMP00000229886 antibody
    • Ribosomal protein S3 antibody
    • rps3 antibody
    • RS3_HUMAN antibody
    • S3 antibody
    • wu:fb13d09 antibody
    • zgc:56088 antibody
    see all

Anti-RPS3 antibody images

  • Anti-RPS3 antibody (ab77330) at 5 µg/ml + immunogen at 0.2 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-RPS3 antibody (ab77330) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

References for Anti-RPS3 antibody (ab77330)

This product has been referenced in:
  • Sengupta T  et al. Base excision repair AP endonucleases and mismatch repair act together to induce checkpoint-mediated autophagy. Nat Commun 4:2578 (2013). Read more (PubMed: 24154628) »
  • Covarrubias S  et al. Coordinated destruction of cellular messages in translation complexes by the gammaherpesvirus host shutoff factor and the Mammalian exonuclease xrn1. PLoS Pathog 7:e1002339 (2011). WB ; Human . Read more (PubMed: 22046136) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HCT116)
Permeabilization Yes - 0.25% Triton X-100
Specification HCT116
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Daniel Scott

Verified customer

Submitted Jan 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"