Overview

  • Product name
    Anti-RPS3A antibody [EPR11982(B)]
    See all RPS3A primary antibodies
  • Description
    Rabbit monoclonal [EPR11982(B)] to RPS3A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RPS3A aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P61247

  • Positive control
    • HeLa, 293T, HepG2 and A431 cell lysates; Human brain and pancreas tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11982(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
ICC/IF 1/50 - 1/100.
IHC-P 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling RPS3A using ab171742 at 1/250 dilution.

    • Immunohistochemistry of paraffin-embedded Human brain tissue labeling RPS3A using ab171742 at 1/250 dilution.

    • All lanes : Anti-RPS3A antibody [EPR11982(B)] (ab171742) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 30 kDa

    References

    This product has been referenced in:
    • Wu Y  et al. Sp110 enhances macrophage resistance toMycobacterium tuberculosisvia inducing endoplasmic reticulum stress and inhibiting anti-apoptotic factors. Oncotarget 8:64050-64065 (2017). Read more (PubMed: 28969051) »
    • Rivera C  et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res 43:9097-106 (2015). WB ; Human . Read more (PubMed: 26405197) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171742.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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