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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
1/500 - 1/1000.
Anti-RPS4Y1 antibody images
Western blot - RPS4Y1 antibody (ab74709)
All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution Lane 1 : Extracts from COLO cells with no peptide Lane 2 : Extracts from HeLa cells with no peptide Lane 3 : Extracts from HUVEC cells with no peptide Lane 4 : Extracts from HUVEC cells with peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 29 kDa Observed band size : 29 kDa Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - RPS4Y1 antibody (ab74709)
Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.
References for Anti-RPS4Y1 antibody (ab74709)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"