Anti-RPS5 antibody (ab168823)
Key features and details
- Rabbit polyclonal to RPS5
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPS5 antibody
See all RPS5 primary antibodies -
Description
Rabbit polyclonal to RPS5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human RPS5 aa 1-100.
Database link: P46782 -
Positive control
- WB: 293T, HeLa, Jurkat, Mouse TCMK-1 and Mouse NIH3T3 whole cell lysates. IP: HeLa cell lysates. IHC-P: Human prostate carcinoma tissue; Mouse teratoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 23 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 23 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
RPS5 is a component of the small 40S subunit of ribosomes. The protein belongs to the S7P family of ribosomal proteins and is found in the cytoplasm. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 456347 Chimpanzee
- Entrez Gene: 506229 Cow
- Entrez Gene: 476366 Dog
- Entrez Gene: 101132936 Gorilla
- Entrez Gene: 6193 Human
- Entrez Gene: 20103 Mouse
- Entrez Gene: 711259 Rhesus monkey
- Omim: 603630 Human
see all -
Alternative names
- 40S ribosomal protein S5 antibody
- 40S ribosomal protein S5 N terminally processed antibody
- Ribosomal protein S5 antibody
see all
Images
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All lanes : Anti-RPS5 antibody (ab168823) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 30 seconds -
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: ab168823 used for IP at 6 µg per reaction (Lane 2). RPS5 was also immunoprecipitated by a previous lot of this antibody (Lane 1). For blotting immunoprecipitated RPS5 ab168823 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
All lanes : Anti-RPS5 antibody (ab168823) at 6 mg/ml
All lanes : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) -
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate carcinoma labeling RPS5 with ab168823 at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab168823 has been referenced in 1 publication.
- Wang YJ et al. Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast. PLoS Biol 16:e2005903 (2018). PubMed: 30208026