Anti-RPS6 antibody - Aminoterminal end (ab63053)

Overview

  • Product nameAnti-RPS6 antibody - Aminoterminal end
    See all RPS6 primary antibodies
  • Description
    Rabbit polyclonal to RPS6 - Aminoterminal end
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N terminal region of human RPS6, conjugated to KLH.

  • Positive control
    • Human colon carcinoma tissue, HL60 cell line lysates and 293 cell lysates transiently transfected with the RPS6 gene.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab63053 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ELISA 1/1000.
IHC-P 1/50.

Target

  • FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
  • Sequence similaritiesBelongs to the ribosomal protein S6e family.
  • Post-translational
    modifications
    Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S6 antibody
    • Air8 antibody
    • NP33 antibody
    • Phosphoprotein NP33 antibody
    • Pp30 antibody
    • Ribosomal protein S6 antibody
    • RP S6 antibody
    • rps6 antibody
    • RS6 antibody
    • RS6_HUMAN antibody
    • S6 antibody
    • S6 Ribosomal Protein antibody
    see all

Anti-RPS6 antibody - Aminoterminal end images

  • Anti-RPS6 antibody - Aminoterminal end (ab63053) at 1/60 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • All lanes : Anti-RPS6 antibody - Aminoterminal end (ab63053) at 1/60 dilution

    Lane 1 : 293 cell lysates nontransfected
    Lane 2 : 293 cell lysates transiently transfected with the RPS6 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ab63053, at 1/50 dilution, staining RPS6 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by immunohistochemistry. ab63053 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-RPS6 antibody - Aminoterminal end (ab63053)

ab63053 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"