Anti-RPS6 (phospho S235 + S236) antibody (ab65750)

Overview

  • Product nameAnti-RPS6 (phospho S235 + S236) antibody
    See all RPS6 primary antibodies
  • Description
    Sheep polyclonal to RPS6 (phospho S235 + S236)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: CAKRRRLSPSPLRA conjugated to KLH, corresponding to C terminal amino acids 229-239 of Human RPS6

  • Positive control
    • Raji cells untreated, or treated with 10 µg/ml S6 inhibitor for 30 min.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

Anti-RPS6 (phospho S235 + S236) antibody images

  • All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab65750) at 2 µg/ml

    Lane 1 : Raji cell extracts, untreated
    Lane 2 : Raji cell extracts, treated with 10 µg/ml S6 inhibitor for 30 min


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RPS6 (phospho S235 + S236) antibody (ab65750)

ab65750 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"