Anti-RPS6 (phospho S240 + S244) antibody (ab53024)

Overview

  • Product nameAnti-RPS6 (phospho S240 + S244) antibody
    See all RPS6 primary antibodies
  • Description
    Rabbit polyclonal to RPS6 (phospho S240 + S244)
  • SpecificityThis antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 240 or serine 244.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 240 or serine 244 (SP-T-S-K-SP)

  • Positive control
    • Extracts from 293 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ELISA 1/20000.

Target

Anti-RPS6 (phospho S240 + S244) antibody images

  • All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab53024) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with serum (10%, 15mins) with no immunizing peptide
    Lane 2 : Extracts from 293 cells treated with serum (10%, 15mins) with immunizing peptide


    Predicted band size : 30 kDa

References for Anti-RPS6 (phospho S240 + S244) antibody (ab53024)

ab53024 has not yet been referenced specifically in any publications.

Product Wall



Unfortunately, we have not tested this antibody in mouse samples and therefore we do not have a western blot with which to compare yours to. As for the identity of the extra band, we have never seen this band before and when I performed a lit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"