Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

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Overview

  • Product name
    Anti-RPS6 (phospho S240 + S244) antibody
    See all RPS6 primary antibodies
  • Description
    Sheep polyclonal to RPS6 (phospho S240 + S244)
  • Host species
    Sheep
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
  • Immunogen

    Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
    Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
    All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml

    Lane 1 : HEK293 cell extracts, serum starved
    Lane 2 : HEK293 cell extracts, 20% new born calf serum
    treated for 30 min.

    Predicted band size: 30 kDa
    Observed band size: 32 kDa (why is the actual band size different from the predicted?)

References

ab65748 has not yet been referenced specifically in any publications.

Publishing research using ab65748? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RPS6 (phospho S240 + S244) antibody

 Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Thermo Scientific Starting Fisher #PI-37539 Block T20 Blocking Buffer as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample
Human Tissue sections (Liver Biopsies)
Specification
Liver Biopsies
Permeabilization
No
Fixative
Paraformaldehyde
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Submitted Nov 14 2013

Western blot abreview for Anti-RPS6 (phospho S240 + S244) antibody

 Average
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Zebrafish Tissue lysate - whole (whole fish)
Specification
whole fish
Treatment
4% glucose 48hrs 4% fructose 48 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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