Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

Overview

  • Product nameAnti-RPS6 (phospho S240 + S244) antibody
    See all RPS6 primary antibodies
  • Description
    Sheep polyclonal to RPS6 (phospho S240 + S244)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6

  • Positive control
    • Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).

Target

Anti-RPS6 (phospho S240 + S244) antibody images

  • All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml

    Lane 1 : HEK293 cell extracts, serum starved
    Lane 2 : HEK293 cell extracts, 20% new born calf serum treated for 30 min.


    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

ab65748 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Thermo Scientific Starting Fisher #PI-37539 Block T20 Blocking Buffer as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample Human Tissue sections (Liver Biopsies)
Specification Liver Biopsies
Permeabilization No
Fixative Paraformaldehyde
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Submitted Nov 14 2013

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Zebrafish Tissue lysate - whole (whole fish)
Specification whole fish
Treatment 4% glucose 48hrs 4% fructose 48 hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"