- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-RPS6 (phospho S240 + S244) antibody
See all RPS6 primary antibodies - DescriptionSheep polyclonal to RPS6 (phospho S240 + S244)
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
- Immunogen
Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6
- Positive control
- Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 150mM Sodium chloride, 10mM HEPES, pH 7.5 -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Applications
Our Abpromise guarantee covers the use of ab65748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa). |
Target
- FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
- Sequence similaritiesBelongs to the ribosomal protein S6e family.
- Post-translational
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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Database links
- Entrez Gene: 396148 Chicken
- Entrez Gene: 787914 Cow
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 29304 Rat
- Entrez Gene: 380281 Xenopus laevis
- Omim: 180460 Human
see all -
Alternative names
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
Anti-RPS6 (phospho S240 + S244) antibody images
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Western blot - RPS6 (phospho S240 + S244) antibody (ab65748)All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml
Lane 1 : HEK293 cell extracts, serum starved
Lane 2 : HEK293 cell extracts, 20% new born calf serum treated for 30 min.
Predicted band size : 30 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
ab65748 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"