The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.
Tissue specificityHighly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 MIT domain. Contains 2 protein kinase domains. Contains 1 PX (phox homology) domain.
DomainThe first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.
Cellular localizationCytoplasm. Membrane. Also found in some small dot-like or ring-shaped early endosome structures.