Anti-Rpt1 antibody (ab22678)

Overview

• Product name
  Anti-Rpt1 antibody
  See all Rpt1 primary antibodies
• Description
  Rabbit polyclonal to Rpt1
• Specificity
  At optimal dilution there is no cross-reactivity with other members of the YTA family.
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Saccharomyces cerevisiae, Schizosaccharomyces pombe
  
• Immunogen

  Recombinant (His-tagged) fusion protein:

  MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR

  LGEEHPLQVA, corresponding to amino acids 1-100 of Rpt1 (yeast).

  Run BLAST with Run BLAST with
• General notes
  Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: None
  Constituents: Whole serum
  
  Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
  
• Purity
  Whole antiserum
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • ATPases

  • Microbiology
  • Organism
  • Eukaryotic Microorganism
  • Yeast
  • Saccharomyces spp

  • Metabolism
  • Types of disease
  • Cancer

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Relevance
  Rpt1 in yeast is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p. In the yeast S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their relationship with TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family (yeast tat binding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length.
• Cellular localization
  Cytoplasmic and Nuclear
• Database links
  • Entrez Gene: 853712 Saccharomyces cerevisiae
  • Entrez Gene: 2539862 Schizosaccharomyces pombe
  • SwissProt: P33299 Saccharomyces cerevisiae
  • SwissProt: O42931 Schizosaccharomyces pombe
• Alternative names
  • 26S protease regulatory subunit 7 homolog antibody
  • CIM5 antibody
  • Protein CIM5 antibody

  • TAT-binding homolog 3 antibody
  • YTA3 antibody

  see all

Protocols

• Western blot protocols