Overview

  • Product nameAnti-Rpt1 antibody
    See all Rpt1 primary antibodies
  • Description
    Rabbit polyclonal to Rpt1
  • SpecificityAt optimal dilution there is no cross-reactivity with other members of the YTA family.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae, Schizosaccharomyces pombe
  • Immunogen

    Recombinant (His-tagged) fusion protein:

    MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR

    LGEEHPLQVA, corresponding to amino acids 1-100 of Rpt1 (yeast).

  • General notesDilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Properties

Applications

Our Abpromise guarantee covers the use of ab22678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa.

Target

  • RelevanceRpt1 in yeast is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p. In the yeast S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their relationship with TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family (yeast tat binding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
    • Alternative names
      • 26S protease regulatory subunit 7 homolog antibody
      • CIM5 antibody
      • Protein CIM5 antibody
      • TAT-binding homolog 3 antibody
      • YTA3 antibody
      see all

    Anti-Rpt1 antibody images

    • Anti-Rpt1 antibody (ab22678) at 1/25000 dilution + Purified yeast 26S proteasome preparation
      Developed using the ECL technique

      Exposure time : 1 minute

    References for Anti-Rpt1 antibody (ab22678)

    ab22678 has not yet been referenced specifically in any publications.

    Product Wall

    Please find below some information about the testing conditions:

    Western blot - Single dimension SDS-PAGE of a yeast whole cell extract and purified yeast 26S proteasome followed by Western blotting shows a major band at ˜52kDa. The antibody...

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    Thank you for contacting Abcam regarding ab22678 and for taking the time to provide some details of the customer's Western blot experiments. I am very sorry to hear that he/she is having problem with this particular product. Here I am forwarding my...

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