- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Rpt1 antibody
See all Rpt1 primary antibodies -
DescriptionRabbit polyclonal to Rpt1
-
Host speciesRabbit
-
SpecificityAt optimal dilution there is no cross-reactivity with other members of the YTA family.
-
Tested applicationsSuitable for: WBmore details
-
Species reactivityReacts with: Saccharomyces cerevisiae, Schizosaccharomyces pombe
-
Immunogen
Recombinant (His-tagged) fusion protein:
MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR
LGEEHPLQVA, corresponding to amino acids 1-100 of Rpt1 (yeast). -
General notesDilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: None
Constituents: Whole serum
Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
-
PurityWhole antiserum
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab22678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 52 kDa. |
Target
-
RelevanceRpt1 in yeast is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and the E3 ubiquitin-protein ligase Ubr1p. In the yeast S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their relationship with TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family (yeast tat binding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length.
-
Cellular localizationCytoplasmic and Nuclear
-
Database links
- Entrez Gene: 853712 Saccharomyces cerevisiae
- Entrez Gene: 2539862 Schizosaccharomyces pombe
- SwissProt: P33299 Saccharomyces cerevisiae
- SwissProt: O42931 Schizosaccharomyces pombe
-
Alternative names
- 26S protease regulatory subunit 7 homolog antibody
- CIM5 antibody
- Protein CIM5 antibody
see all
Images
-
Western blot - Anti-Rpt1 antibody (ab22678)Anti-Rpt1 antibody (ab22678) at 1/25000 dilution + Purified yeast 26S proteasome preparation
Developed using the ECL technique.
Exposure time: 1 minute
Protocols
References
ab22678 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"