The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/10000 - 1/50000. Detects a band of approximately 48 kDa.
Notes: Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceIn the yeast, S. cerevisiae, 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their relationship with TBP-1, a human immunodeficiency tat-binding protein, they have been designated the YTA family (yeast tat-binding analogues). The most outstanding feature of the YTA proteins and their relatives is that they share a region of high similarity which extends to some 300 amino acids in length. The amino acid sequences preceding the region of high similarity are less well conserved but do contain similar structural domains, i.e. coil-coil propensities, leucine zipper-like structures. However, despite the highly conserved structural features among the YTA family, diverse functions have been assigned to the various family members. The putative gene products of YTA1, YTA2, YTA3 and YTA5 are all closely related to proteins known to be constituents of the human 26S proteasome. RPT2 (YTA5), of all members of the YTA family, shows the highest similarity with subunit 4 of the human 26S proteasome, and is presumed to be the true yeast homologue.