The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Notes: Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceIn the yeast S. cerevisiae 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their similarities to TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family or yeast tat binding analogues. All share a region of high similarity of approximately 300 amino acids in length. Rpt5p or YTA1 ,is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates.