Recombinant
RabMAb

Anti-RPUSD3 antibody [EPR12911] (ab181121)

Overview

  • Product name
    Anti-RPUSD3 antibody [EPR12911]
    See all RPUSD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12911] to RPUSD3
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human RPUSD3 aa 1-150. The exact sequence is proprietary.
    Database link: Q6P087

  • Positive control
    • U87-MG, SH-SY5Y, 293T or Human fetal brain lysate, SH-SY5Y or U87-MG cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12911
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab181121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 38 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/290. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Flow cytometry analysis of U87-MG cells using ab181121 at a 1/290 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunofluorescence analysis of SH-SY5Y cells (fixative 4% paraformaldehyde) labeling RPUSD3 with ab181121 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

  • All lanes : Anti-RPUSD3 antibody [EPR12911] (ab181121) at 1/1000 dilution

    Lane 1 : U87-MG lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : 293T lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 38 kDa

References

ab181121 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181121.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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