Overview

  • Product name
    Anti-RPUSD4 antibody
  • Description
    Rabbit polyclonal to RPUSD4
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: NLVVINKPYG LPVHGGPGVQ LCITDVLPIL AKMLHGHKAE PLHLCHRLDK ETTGVMVLAW DKDMAHQVQE LFRTRQVVKK YWAITVHVP, corresponding to internal sequence amino acids 105-193 of Human RPUSD4.

  • Positive control
    • Human colon tissue; RPUSD4 over-expression HEK293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122571 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T cell lysate
    Lane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells

    Developed using the ECL technique

References

This product has been referenced in:
  • Zaganelli S  et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). Read more (PubMed: 28082677) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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