Overview

  • Product name
  • Description
    Rabbit polyclonal to RRAD
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human RRAD.

  • Positive control
    • Human heart tissue. Extracts from HeLa cells, HepG2 cells and MCF-7 cells. A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
  • Tissue specificity
    Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
  • Sequence similarities
    Belongs to the small GTPase superfamily. RGK family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTP binding protein RAD antibody
    • GTP-binding protein RAD antibody
    • RAD antibody
    • RAD_HUMAN antibody
    • RAD1 antibody
    • RAS (RAD and GEM) like GTP binding 3 antibody
    • Ras associated with diabetes antibody
    • Ras related associated with diabetes antibody
    • REM3 antibody
    • RRAD antibody
    see all

Images

  • All lanes : Anti-RRAD antibody (ab75100) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from MCF-7 cells
    Lane 4 : Extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of RRAD in paraffin-embedded human heart tissue using ab75100, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of RRAD in A549 cells using ab75100, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:
  • Yan Y  et al. Ras-related associated with diabetes gene acts as a suppressor and inhibits Warburg effect in hepatocellular carcinoma. Onco Targets Ther 9:3925-37 (2016). IHC . Read more (PubMed: 27418837) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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