Overview

  • Product nameAnti-RRAGB antibody
    See all RRAGB primary antibodies
  • Description
    Mouse monoclonal to RRAGB
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-347 of Human RRAGB

Properties

Applications

Our Abpromise guarantee covers the use of ab56633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGuanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway.
    • Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
    • Cellular localizationCytoplasm. Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • GTP-binding protein ragB antibody
      • Rag B antibody
      • RagB antibody
      • Ras-related GTP-binding protein B antibody
      • RRAGB antibody
      • RRAGB_HUMAN antibody
      see all

    Anti-RRAGB antibody images

    • Western blot against tagged recombinant protein immunogen using ab56633 RRAGB antibody at 1ug/ml. Predicted band size of immunogen is 64 kDa

    References for Anti-RRAGB antibody (ab56633)

    This product has been referenced in:
    • Sancak Y  et al. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science 320:1496-501 (2008). WB ; Human . Read more (PubMed: 18497260) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa, 293T)
    Loading amount 15 µg
    Specification HeLa, 293T
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Nov 18 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"