Immunocytochemistry/ Immunofluorescence - Anti-RRAS antibody (ab47536)Image from Miller JP et al., PLoS Genet 8(11): e1003042.. Fig 6.; doi: 10.1371/journal.pgen.1003042. Epub 2012 Nov 29.
Immunofluorescence analysis of Hdh111Q/111Q cells, staining RRAS with ab47536.
Cells were fixed in 4% paraformaldehyde, permeabilized in 0.25% Triton X-100 and blocked for 1 hr at room-temp with 10% normal goat serum. Cells were incubated with primary antibody (1/250 in 1% BSA in TBS) overnight at 4°C. An AlexaFluor®555-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).
References for Anti-RRAS antibody (ab47536)
This product has been referenced in:
Miller JP et al. A genome-scale RNA-interference screen identifies RRAS signaling as a pathologic feature of Huntington's disease. PLoS Genet8:e1003042 (2012).
WB, ICC/IF, IHC-P
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