Anti-RRAS antibody (ab82356)
Key features and details
- Rabbit polyclonal to RRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RRAS antibody
See all RRAS primary antibodies -
Description
Rabbit polyclonal to RRAS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human RRAS aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93071) -
Positive control
- Recombinant Human RRAS protein (ab114612) can be used as a positive control in WB. This antibody gave a positive signal in human skeletal muscle as well as the following whole cell lysates: HeLa; HT29; DU145; PC3; A549.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
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Notes |
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WB
1/250. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Regulates the organization of the actin cytoskeleton. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Cellular localization
Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine. - Information by UniProt
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Database links
- Entrez Gene: 6237 Human
- Omim: 165090 Human
- SwissProt: P10301 Human
- Unigene: 515536 Human
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Alternative names
- Oncogene RRAS antibody
- p23 antibody
- R Ras antibody
see all
Images
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All lanes : Anti-RRAS antibody (ab82356) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HT29 (Human colon adenocarcinoma) Whole Cell Lysate
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 5 : PC-3 whole cell lysate (ab3954)
Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 63 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab82356 has not yet been referenced specifically in any publications.