Recombinant
RabMAb

Anti-RRAS [EPR15843] antibody (ab191399)

Overview

  • Product name
    Anti-RRAS [EPR15843] antibody
    See all RRAS primary antibodies
  • Description
    Rabbit monoclonal [EPR15843] to RRAS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RRAS aa 1-100. The exact sequence is proprietary.
    Database link: P10301

  • Positive control
    • Human fetal kidney and heart lysates, HT29 and A549 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Flow Cyt 1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Regulates the organization of the actin cytoskeleton.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Ras family.
  • Cellular localization
    Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine.
  • Information by UniProt
  • Database links
  • Alternative names
    • Oncogene RRAS antibody
    • p23 antibody
    • R Ras antibody
    • Ras related Protein antibody
    • Ras related protein R Ras antibody
    • Ras-related protein R-Ras antibody
    • related RAS viral (r ras) oncogene homolog antibody
    • RRAS antibody
    • RRAS_HUMAN antibody
    see all

Images

  • All lanes : Anti-RRAS [EPR15843] antibody (ab191399) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HT29 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Anti-RRAS [EPR15843] antibody (ab191399) at 1/10000 dilution + A549 cell lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Flow cytometry analysis of paraformaldehyde-fixed A549 cells labeling RRAS with ab191399 at a 1/350 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

References

ab191399 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191399.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up