The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use a concentration of 5 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.
Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas.
Involvement in disease
Belongs to the small GTPase superfamily. Ras family.
May be post-translationally modified by both palmitoylation and polyisoprenylation.
Cell membrane. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
Related RAS viral (r ras) oncogene homolog 2 antibody
Related RAS viral oncogene homolog 2 antibody
RRAS 2 antibody
TC 21 antibody
Teratocarcinoma oncogene antibody
Teratocarcinoma oncogene TC21 antibody
Anti-RRAS2 antibody images
Flow Cytometry - Anti-RRAS2 antibody (ab56859)
Overlay histogram showing A431 cells stained with ab56859 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56859, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
IHC-P - RRAS2 antibody (ab56859)
RRAS2 antibody (ab56859) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human dysgerminoma tissue.
Western blot - RRAS2 antibody (ab56859)
RRAS2 antibody (ab56859) at 1ug/lane + A-431 cell lysate at 25ug/lane.