Anti-RRBP1 antibody (ab224354)
Key features and details
- Rabbit polyclonal to RRBP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RRBP1 antibody
See all RRBP1 primary antibodies -
Description
Rabbit polyclonal to RRBP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human RRBP1 aa 900-1050.
Database link: Q9P2E9 -
Positive control
- WB: Caco-2 cell lysate. IHC-P: Human placenta and stomach tissue. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 152 kDa.
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IHC-P |
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 152 kDa. |
IHC-P
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6238 Human
- Omim: 601418 Human
- SwissProt: Q9P2E9 Human
- Unigene: 472213 Human
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Alternative names
- 180 kDa ribosome receptor homolog antibody
- DKFZp586A1420 antibody
- ES/130 antibody
see all
Images
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Paraffin-embedded human stomach tissue stained for RRBP1 using ab224354 at 1/2500 in immunohistochemical analysis.
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Anti-RRBP1 antibody (ab224354) at 1/1000 dilution + Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 152 kDa -
Paraffin-embedded human placenta tissue stained for RRBP1 using ab224354 at 1/2500 in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for RRBP1 (greeen) using ab224354 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224354 has been referenced in 1 publication.
- Zhou H et al. Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma. Aging (Albany NY) 13:5312-5331 (2021). PubMed: 33535184