Overview

  • Product nameAnti-RRM1 antibody
    See all RRM1 primary antibodies
  • Description
    Rabbit polyclonal to RRM1
  • Tested applicationsSuitable for: WB, ICC, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C-terminus of human RRM1.

  • Positive control
    • Human placenta.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab81085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ICC Use at an assay dependent concentration. PubMed: 23840376
IP Use at an assay dependent concentration.
IHC-P 1/100.

Target

  • FunctionProvides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
  • PathwayGenetic information processing; DNA replication.
  • Sequence similaritiesBelongs to the ribonucleoside diphosphate reductase large chain family.
    Contains 1 ATP-cone domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • R1 antibody
    • Ribonucleoside diphosphate reductase large subunit antibody
    • Ribonucleoside diphosphate reductase M1 chain antibody
    • Ribonucleoside diphosphate reductase subunit M1 antibody
    • Ribonucleoside reductase, large subunit antibody
    • Ribonucleoside-diphosphate reductase large subunit antibody
    • Ribonucleoside-diphosphate reductase subunit M1 antibody
    • Ribonucleotide reductase large chain antibody
    • Ribonucleotide reductase large subunit antibody
    • Ribonucleotide reductase M1 antibody
    • Ribonucleotide reductase M1 polypeptide antibody
    • Ribonucleotide reductase R1 subunit antibody
    • Ribonucleotide reductase, M1 subunit antibody
    • RIR 1 antibody
    • RIR1 antibody
    • RIR1_HUMAN antibody
    • RR 1 antibody
    • RR1 antibody
    • RRM 1 antibody
    • RRM1 antibody
    see all

Anti-RRM1 antibody images

  • ab81085, at 1/100 dilution, staining RRM1 in Human Placenta by Immunohistochemistry using formalin-fixed paraffin-embedded tissue.
  • Anti-RRM1 antibody (ab81085) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • RRM1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RRM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81085.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
    Band: Band: 90kDa: RRM1.
  • Immunohistochemical analysis of Human pancreatic ductal adenocarcinoma tissue, staining RRM1 with ab81085 at 1/50 dilution.

References for Anti-RRM1 antibody (ab81085)

This product has been referenced in:
  • Szulkin A  et al. Variation in drug sensitivity of malignant mesothelioma cell lines with substantial effects of selenite and bortezomib, highlights need for individualized therapy. PLoS One 8:e65903 (2013). ICC ; Human . Read more (PubMed: 23840376) »
  • Valsecchi ME  et al. Is there a role for the quantification of RRM1 and ERCC1 expression in pancreatic ductal adenocarcinoma? BMC Cancer 12:104 (2012). IHC-P ; Human . Read more (PubMed: 22436573) »

See all 4 Publications for this product

Product Wall

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab81085 on the order ***, which should arrive on Tuesday.

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Thank you very much for your interest in our RRM1 antibodies.

While we have not tested any of our antibodies for use on mouse samples, the mouse and human RRM1 proteins are 98% homologous. Therefore, I can offer a discount off a future purch...

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Thank you for your call today and for your patience while I've been in touch with the lab about this antibody.

The concentration of the lot you're using is 0.2 mg/mL.

I hope this information isuseful, but please let me know if you h...

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Thank you for your telephone enquiry yesterday.

I can confirm that the concentration of is lot specific. The concentration of lotGR-76187-1 is 0.2 mg/ml.

I hope this will be helpful to you. If you have any further questions, pleas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"