Overview

  • Product name
  • Description
    Mouse monoclonal to RRM2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
  • Pathway
    Genetic information processing; DNA replication.
  • Sequence similarities
    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Post-translational
    modifications
    Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cb111 antibody
    • chunp6884 antibody
    • etID309896.20 antibody
    • R2 antibody
    • reductase M2 polypeptide variant 1 antibody
    • reductase M2 polypeptide variant 2 antibody
    • reductase M2 polypeptide variant 3a antibody
    • reductase M2 polypeptide variant 3b antibody
    • reductase M2 polypeptide variant 3c antibody
    • reductase M2 polypeptide variant 3d antibody
    • Ribonucleoside-diphosphate reductase subunit M2 antibody
    • Ribonucleotide reductase M2 antibody
    • Ribonucleotide reductase M2 polypeptide antibody
    • Ribonucleotide reductase M2 subunit antibody
    • ribonucleotide reductase protein r2 class I antibody
    • Ribonucleotide reductase regulatory subunit M2 antibody
    • Ribonucleotide reductase small chain antibody
    • Ribonucleotide reductase small subunit antibody
    • Ribonucleotide reductase, R2 subunit antibody
    • RIR2_HUMAN antibody
    • RR2 antibody
    • RR2M antibody
    • RRM2 antibody
    see all

Images

  • RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 45 kDa
  • ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Tu M  et al. Vasohibin 2 reduces chemosensitivity to gemcitabine in pancreatic cancer cells via Jun proto-oncogene dependent transactivation of ribonucleotide reductase regulatory subunit M2. Mol Cancer 16:66 (2017). Read more (PubMed: 28327155) »
  • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 20 2014

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
African Green Monkey Cell lysate - whole cell (COS-7 - AGM kidney tissue fibroblast like line)
Specification
COS-7 - AGM kidney tissue fibroblast like line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2014

Merci de nous avoir contactés. Nous serons ravis de vous envoyer un code de test pour des anticorps anti-RPS23, anti-pumilio2 et anti-RRM2. Avant l'édition de ces codes, je vous invite à consulter les listes des six anti-pumilio2 et des quatre anti-RRM...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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