Overview

  • Product nameAnti-RRN3 antibody
    See all RRN3 primary antibodies
  • Description
    Rabbit polyclonal to RRN3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    A region within synthetic peptide: KKDIVEDEDD DFLKGEVPQN DTVIGITPSS FDTHFRSPSS SVGSPPVLYM, corresponding to amino acids 597-646 of Human RRN3

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab42539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRequired for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
  • Sequence similaritiesBelongs to the RRN3 family.
  • Post-translational
    modifications
    Phosphorylation is required for participation in rDNA transcription.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL023001 antibody
    • DKFZp566E104 antibody
    • E130302O19Rik antibody
    • MGC104238 antibody
    • R75565 antibody
    • RGD1305001 antibody
    • RNA polymerase I-specific transcription initiation factor RRN3 antibody
    • Rrn3 antibody
    • RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) antibody
    • RRN3_HUMAN antibody
    • TIF-IA antibody
    • TIF1A antibody
    • TIFIA antibody
    • Transcription Initiation Factor 1A antibody
    • Transcription initiation factor IA antibody
    • Transcription initiation factor TIF IA antibody
    see all

Anti-RRN3 antibody images

  • Anti-RRN3 antibody (ab42539) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-RRN3 antibody (ab42539)

ab42539 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab42539.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"