The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
FunctionRequired for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
Sequence similaritiesBelongs to the RRN3 family.
Post-translational modificationsPhosphorylation is required for participation in rDNA transcription.