Overview

  • Product name
  • Description
    Rabbit polyclonal to RRP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Opossum, Common marmoset, Orangutan, Xenopus tropicalis, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 243-293 of Human RRP4 (NP_055100.2).

  • Positive control
    • HeLa, Jurkat, NIH 3T3 and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab156698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
  • Cellular localization
    Cytoplasmic, Exosome and Nuclear
  • Database links
  • Alternative names
    • EXOSC2 antibody
    • Exosome complex exonuclease RRP4 antibody
    • Exosome component 2 antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4) antibody
    • Homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease antibody
    • hRrp4p antibody
    • OTTHUMP00000022370 antibody
    • OTTHUMP00000022371 antibody
    • OTTHUMP00000022372 antibody
    • OTTHUMP00000022373 antibody
    • OTTHUMP00000022374 antibody
    • p7 antibody
    • Ribosomal RNA processing protein 4 antibody
    • RRP4 antibody
    • Rrp4p antibody
    see all

Images

  • All lanes : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml

    Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
    Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 3 minutes
  • Lane 1 : Alternative anti-RRP4 antibody
    Lane 2 : Anti-RRP4 antibody (ab156698) at 0.4 µg/ml
    Lane 3 : Control IgG

    All lanes : HEK293T whole cell lysate

References

This product has been referenced in:
  • McIver SC  et al. Exosome complex orchestrates developmental signaling to balance proliferation and differentiation during erythropoiesis. Elife 5:N/A (2016). IP . Read more (PubMed: 27543448) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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