The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Use a concentration of 4 - 8 µg/ml.
RelevanceThe exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
Cellular localizationCytoplasmic, Exosome and Nuclear
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling RRP4 with ab66666 at 4.0-8.0µg/ml. Positive staining shown in alveolar cells. Magnification: 400X.
Western blot - RRP4 antibody (ab66666)
All lanes : Anti-RRP4 antibody (ab66666) at 1.25 µg/ml
Lane 1 : Marker Lane 2 : Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 33 kDa Observed band size : 33 kDa Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
References for Anti-RRP4 antibody (ab66666)
has not yet been referenced specifically in any publications.
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