The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/40 - 1/60.
1/50 - 1/100.
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism.
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181211 at 1/50 dilution. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling RRP4 with ab181211 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RRP4 with ab181211 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-RRP4 [EPR13605] antibody (ab181211)
All lanes : Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211) at 1/50000 dilution
Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution