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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
Anti-RRP7A antibody images
Immunocytochemistry/ Immunofluorescence - Anti-RRP7A antibody (ab121142)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121142 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP7A antibody (ab121142)
Immunohistochemical staining of Human lateral ventricle with ab121142 1/150 dilution. The staining shows nucleolar positivity in neuronal cells and nuclear positivity in glial cells.
Western blot - Anti-RRP7A antibody (ab121142)
All lanes : Anti-RRP7A antibody (ab121142) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A-431 cell lysate
Lane 4 : Liver cell lysate
Lane 5 : Tonsil cell lysate
References for Anti-RRP7A antibody (ab121142)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"