Overview

  • Product name(R,S)-3,5-DHPG
  • Description
    Group I mGlu receptor agonist
  • Biological descriptionSelective group I mGlu receptor agonist

    Also available in simple stock solutions (ab146669) - add 1 ml of water to get an exact, ready-to-use concentration.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120020 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    (R,S)-3,5-DHPG images

    • ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for (R,S)-3,5-DHPG (ab120020)

    This product has been referenced in:
    • Lin FF  et al. Cloning and stable expression of the mGluR1b subtype of human metabotropic receptors and pharmacological comparison with the mGluR5a subtype. Neuropharmacology 36:917-31 (1997). Read more (PubMed: 9257936) »
    • Brabet I  et al. Phenylglycine derivatives discriminate between mGluR1- and mGluR5-mediated responses. Neuropharmacology 34:895-903 (1995). Read more (PubMed: 8532171) »
    • Ito I  et al. 3,5-Dihydroxyphenyl-glycine: a potent agonist of metabotropic glutamate receptors. Neuroreport 3:1013-6 (1992). Read more (PubMed: 1362358) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"