Overview

  • Product name(R,S)-Rolipram
  • Description
    Selective PDE4 inhibitor
  • Biological descriptionSelective cAMP-specific phosphodiesterase PDE4 inhibitor (IC50 values are 1.1 and 0.17 μM for inhibition of mouse PDE4A1 and PDE4B, respectively)
  • Purity> 99%

Properties

  • Chemical name(R,S)-4-(3-(Cyclopentyloxy)-4-methoxyphenyl)pyrrolidin-2-one
  • Molecular weight275.35
  • Chemical structureChemical Structure
  • Molecular formulaC16H21NO3
  • CAS Number61413-54-5
  • PubChem identifier5092
  • Storage instructionsStore at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESCOc2ccc(cc2OC1CCCC1)C3CC(=O)NC3
  • Source

    Synthetic

  • Research areas

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab120029 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    (R,S)-Rolipram images

    • ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for (R,S)-Rolipram (ab120029)

    This product has been referenced in:
    • Cherry JA  et al. Diazepam and rolipram differentially inhibit cyclic AMP-specific phosphodiesterases PDE4A1 and PDE4B3 in the mouse. Biochim Biophys Acta 1518:27-35 (2001). Read more (PubMed: 11267656) »
    • Zhu J  et al. The antidepressant and antiinflammatory effects of rolipram in the central nervous system. CNS Drug Rev 7:387-98 (2001). Read more (PubMed: 11830756) »
    • Schneider HH  et al. Stereospecific binding of the antidepressant rolipram to brain protein structures. Eur J Pharmacol 127:105-15 (1986). Read more (PubMed: 3019721) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120029.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"