Biological descriptionSelective cAMP-specific phosphodiesterase PDE4 inhibitor (IC50 values are 1.1 and 0.17 μM for inhibition of mouse PDE4A1 and PDE4B, respectively)
Storage instructionsStore at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Functional Studies - (R,S)-Rolipram (ab120029)
ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature. The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for (R,S)-Rolipram (ab120029)
This product has been referenced in:
Cherry JA et al. Diazepam and rolipram differentially inhibit cyclic AMP-specific phosphodiesterases PDE4A1 and PDE4B3 in the mouse. Biochim Biophys Acta1518:27-35 (2001).
Read more (PubMed: 11267656) »
Zhu J et al. The antidepressant and antiinflammatory effects of rolipram in the central nervous system. CNS Drug Rev7:387-98 (2001).
Read more (PubMed: 11830756) »
Schneider HH et al. Stereospecific binding of the antidepressant rolipram to brain protein structures. Eur J Pharmacol127:105-15 (1986).
Read more (PubMed: 3019721) »