Overview

  • Product name
    Anti-Rsafd1 antibody
  • Description
    Rabbit polyclonal to Rsafd1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 480-662 of Human Rsafd1 (UniProt ID: Q9NV66)

  • Positive control
    • A549, HeLa, HepG2 or HCT116 whole cell lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 84 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.
  • Pathway
    tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
  • Sequence similarities
    Belongs to the TYW1 family.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10900 antibody
    • GC23001 antibody
    • MGC60291 antibody
    • Radical S-adenosyl methionine and flavodoxin domain-containing protein 1 antibody
    • RSAFD1 antibody
    • tRNA wybutosine-synthesizing protein 1 homolog antibody
    • tRNA yW synthesizing protein 1 homolog antibody
    • tRNA-yW-synthesizing protein antibody
    • TYW1 antibody
    • TYW1_HUMAN antibody
    • TYW1A antibody
    • YPL207W antibody
    see all

Images

  • Anti-Rsafd1 antibody (ab154662) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 84 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa, labeling Rsafd1 using ab154662 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154662 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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