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Rabbit polyclonal to Rsafd1
Predicted to work with:
Recombinant fragment, corresponding to a region within amino acids 480-662 of Human Rsafd1 (UniProt ID: Q9NV66)
A549, HeLa, HepG2 or HCT116 whole cell lysate; HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Predicted molecular weight: 84 kDa.
1/100 - 1/1000.
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.
tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
Belongs to the TYW1 family.
Contains 1 flavodoxin-like domain.
Information by UniProt
Western blot - Anti-Rsafd1 antibody (ab154662)
Anti-Rsafd1 antibody (ab154662) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 84 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Rsafd1 antibody (ab154662)
Immunofluorescence analysis of methanol-fixed HeLa, labeling Rsafd1 using ab154662 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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