Anti-RSBN1 antibody - N-terminal (ab170387)

Overview

  • Product name
    Anti-RSBN1 antibody - N-terminal
  • Description
    Rabbit polyclonal to RSBN1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RSBN1 aa 10-38 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5VWQ0

  • Positive control
    • Jurkat cells; Jukat cell line lysate; Human placenta tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170387 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 90 kDa.

Target

  • Relevance
    RSBN1 belongs to the round spermatid basic protein 1 family. The function of this protein remains unknown.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • C230004D03Rik antibody
    • DKFZp781E21150 antibody
    • E330004N01 antibody
    • mKIAA3002 antibody
    • OTTHUMP00000013723 antibody
    • OTTMUSP00000028850 antibody
    • OTTMUSP00000028851 antibody
    • RGD1307231 antibody
    • ROSBIN antibody
    • Rosbin, round spermatid basic protein 1 antibody
    • Round spermatid basic protein 1 antibody
    • RP11 324J2.1 antibody
    • Rsbn antibody
    • Rsbp antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labeling RSBN1 with ab170387 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC conjugated Donkey anti-Rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling RSBN1 with ab170387 at 1/10 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-RSBN1 antibody - N-terminal (ab170387) at 1/100 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size: 90 kDa

References

ab170387 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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