Overview

  • Product nameAnti-RSF1 antibody
    See all RSF1 primary antibodies
  • Description
    Rabbit polyclonal to RSF1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1116-1165 (QDEFVVSDEN PDESEEDPPS NDDSDTDFCS RRLRRHPSRP MRQSRRLRRK) of human RSF1 (NP_057662).

  • Positive control
    • Human fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 163 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceRSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • HBV pX associated protein 8 antibody
    • Hepatitis B virus x associated protein antibody
    • HXBAP antibody
    • p325 antibody
    • p325 subunit of RSF chromatin remodeling complex antibody
    • remodeling and spacing factor 1 antibody
    • RSF 1 antibody
    • XAP8 antibody
    see all

Anti-RSF1 antibody images

  • Anti-RSF1 antibody (ab83322) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 163 kDa
    Observed band size : 168 kDa (why is the actual band size different from the predicted?)

References for Anti-RSF1 antibody (ab83322)

ab83322 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"