• Product name
    Anti-RSF1 antibody [EPR3749(2)]
    See all RSF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3749(2)] to RSF1
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human RSF1.

  • Positive control
    • Jurkat, 293T and HeLa cell lysates; Human colon, endometrial adenocarcinoma and tonsil tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab109002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 164 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      RSF1 is involved in transcription repression when recruited to DNA via the DNA binding of GAL4. It is also involved in chromatin remodeling and spacing when associated with SNF2H and transcription coactivation when associated with hepatitis B virus X protein (HBX). The RSF1 gene is localized to the long arm of human chromosome 11 between q13.4 and q14.1. This region is amplified and rearranged in many tumors, indicating that it plays a role in tumorigenesis similar to that of other PHD-containing proteins.
    • Cellular localization
    • Database links
    • Alternative names
      • HBV pX associated protein 8 antibody
      • Hepatitis B virus x associated protein antibody
      • HXBAP antibody
      • p325 antibody
      • p325 subunit of RSF chromatin remodeling complex antibody
      • remodeling and spacing factor 1 antibody
      • RSF 1 antibody
      • XAP8 antibody
      see all


    • All lanes : Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 164 kDa
    • ab109002 at 1/250 dilution, staining RSF1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109002 at 1/250 dilution, staining RSF1 in Human endometrial adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109002 at 1/250 dilution, staining RSF1 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.


    This product has been referenced in:
    • Lee HS  et al. The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nat Commun 6:7904 (2015). ICC . Read more (PubMed: 26259146) »
    • Ren J  et al. Overexpression of Rsf-1 correlates with pathological type, p53 status and survival in primary breast cancer. Int J Clin Exp Pathol 7:5595-608 (2014). IHC ; Human . Read more (PubMed: 25337201) »

    See all 2 Publications for this product

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