Overview

  • Product nameAnti-RSG1 antibody
    See all RSG1 primary antibodies
  • Description
    Rabbit polyclonal to RSG1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KFDQYMHTDV PERDLTAFRQ AWELPLLRVK SVPGRRLADG RTLDGRAGLA DVAHILNGLA EQLWHQDQVA AGLLPNPPES APE, corresponding to amino acids 176-258 of Human RSG1 (Q9BU20).

  • Positive control
    • Human liver tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionPotential effector of the planar cell polarity signaling pathway. Plays a role in targeted membrane trafficking most probably at the level of vesicle fusion with membranes. Involved in cilium biogenesis by regulating the transport of cargo proteins to the basal body and to the apical tips of cilia. More generally involved in exocytosis in secretory cells.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
  • Cellular localizationCytoplasm > cytoskeleton > cilium basal body.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1orf89 antibody
    • Miro domain containing protein C1orf89 antibody
    • Rem/Rab Similar GTPase 1 antibody
    • REM2- and Rab-like small GTPase 1 antibody
    • RP4-733M16.4 antibody
    • Rsg1 antibody
    • RSG1_HUMAN antibody
    see all

Anti-RSG1 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121990 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121990, at 1/40 dilution, staining RSG1 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References for Anti-RSG1 antibody (ab121990)

ab121990 has not yet been referenced specifically in any publications.

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