Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181)

Overview

  • Product name
    Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell)
  • General notes
    ab94181 is a 293T cell transfected lysate in which Human Rsk 2 / MAPKAP Kinase 1b has been transiently over-expressed using a pCMV-Rsk 2 / MAPKAP Kinase 1b plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Tissue specificity: Expressed in many tissues, highest levels in skeletal muscle. Disease: Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains. PTM: Autophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.

Applications

Our Abpromise guarantee covers the use of ab94181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94181 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b antibody (ab76886) at 1/500 dilution

    Lane 1 : Rsk 2 / MAPKAP Kinase 1b overexpression 293T lysate (whole cell) (ab94181)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94181 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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