The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa.
Use at an assay dependent concentration.
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Expressed in many tissues, highest levels in skeletal muscle.
Involvement in disease
Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains.
Autophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
ab111068 at 20 µg/ml staining Rsk 2 / MAPKAP Kinase 1b in formalin-fixed, paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab111068 at 20 µg/ml staining Rsk 2 / MAPKAP Kinase 1b in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.