FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains.
Post-translational modificationsAutophosphorylated on Ser-386, as part of the activation process. Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Rsk 2 / MAPKAP Kinase 1b with ab90526 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Rsk 2 / MAPKAP Kinase 1b antibody (ab90526)
All lanes : Anti-Rsk 2 / MAPKAP Kinase 1b antibody (ab90526) at 0.4 µg/ml
Lane 1 : whole cell lysate from HeLa cells at 50 µg Lane 2 : whole cell lysate from HeLa cells at 15 µg Lane 3 : whole cell lysate from HeLa cells at 5 µg Lane 4 : whole cell lysate from 293T cells at 50 µg Lane 5 : whole cell lysate from mouse NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 84 kDa
Exposure time : 30 seconds
References for Anti-Rsk 2 / MAPKAP Kinase 1b antibody (ab90526)
has not yet been referenced specifically in any publications.
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