Validated using a knockout cell line
Recombinant
RabMAb

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

Overview

  • Product name
    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83]
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
  • Description
    Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
  • Specificity
    The antibody recognises Rsk2. It does not cross react with other S6 kinase family members.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IP, ChIPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near N-terminus of human RSK2

  • Positive control
    • Hela cell lysate and human lymphoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    Y83
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
ChIP Use at an assay dependent concentration. PubMed: 25368387
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Tissue specificity
      Expressed in many tissues, highest levels in skeletal muscle.
    • Involvement in disease
      Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Autophosphorylated on Ser-386, as part of the activation process.
      Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 3 antibody
      • CLS antibody
      • HU 3 antibody
      • HU2 antibody
      • HU3 antibody
      • Insulin stimulated protein kinase 1 antibody
      • Insulin-stimulated protein kinase 1 antibody
      • ISPK-1 antibody
      • ISPK1 antibody
      • KS6A3_HUMAN antibody
      • MAP kinase activated protein kinase 1b antibody
      • MAP kinase-activated protein kinase 1b antibody
      • MAPK activated protein kinase 1b antibody
      • MAPK-activated protein kinase 1b antibody
      • MAPKAP kinase 1b antibody
      • MAPKAPK 1b antibody
      • MAPKAPK-1b antibody
      • MAPKAPK1B antibody
      • Mental retardation, X linked 19 antibody
      • MRX19 antibody
      • OTTHUMP00000023036 antibody
      • p90 RSK2 antibody
      • p90 RSK3 antibody
      • p90-RSK 3 antibody
      • p90RSK3 antibody
      • pp90RSK2 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
      • Ribosomal protein S6 kinase alpha 3 antibody
      • Ribosomal protein S6 kinase alpha-3 antibody
      • Ribosomal protein s6 kinase ii alpha 2 antibody
      • Ribosomal S6 kinase 2 antibody
      • Rps6ka3 antibody
      • RSK antibody
      • RSK-2 antibody
      • RSK2 antibody
      • S6 kinase 2 antibody
      • S6K alpha3 antibody
      • S6K-alpha-3 antibody
      see all

    Images



    • Predicted band size : 84 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (40 µg)
      Lane 4: (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773
      andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
      secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue
    • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + Hela cell lysate

      Predicted band size : 84 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Takada I DGCR14 induces Il17a gene expression through the ROR?/BAZ1B/RSKS2 complex. Mol Cell Biol 35:344-55 (2015). WB, ChIP ; Mouse . Read more (PubMed: 25368387) »
    • Yu G  et al. RSK promotes prostate cancer progression in bone through ING3, CKAP2, and PTK6-mediated cell survival. Mol Cancer Res 13:348-57 (2015). Read more (PubMed: 25189355) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab32133.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up