Overview

  • Product nameAnti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83]
    See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
  • Description
    Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
  • SpecificityThe antibody recognises Rsk2. It does not cross react with other S6 kinase family members.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IP, ChIPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near N-terminus of human RSK2

  • Positive control
    • Hela cell lysate and human lymphoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberY83
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab32133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
ChIP Use at an assay dependent concentration. PubMed: 25368387
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
    • Tissue specificityExpressed in many tissues, highest levels in skeletal muscle.
    • Involvement in diseaseDefects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Autophosphorylated on Ser-386, as part of the activation process.
      Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa ribosomal protein S6 kinase 3 antibody
      • CLS antibody
      • HU 3 antibody
      • HU2 antibody
      • HU3 antibody
      • Insulin stimulated protein kinase 1 antibody
      • Insulin-stimulated protein kinase 1 antibody
      • ISPK-1 antibody
      • ISPK1 antibody
      • KS6A3_HUMAN antibody
      • MAP kinase activated protein kinase 1b antibody
      • MAP kinase-activated protein kinase 1b antibody
      • MAPK activated protein kinase 1b antibody
      • MAPK-activated protein kinase 1b antibody
      • MAPKAP kinase 1b antibody
      • MAPKAPK 1b antibody
      • MAPKAPK-1b antibody
      • MAPKAPK1B antibody
      • Mental retardation, X linked 19 antibody
      • MRX19 antibody
      • OTTHUMP00000023036 antibody
      • p90 RSK2 antibody
      • p90 RSK3 antibody
      • p90-RSK 3 antibody
      • p90RSK3 antibody
      • pp90RSK2 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 3 antibody
      • Ribosomal protein S6 kinase alpha 3 antibody
      • Ribosomal protein S6 kinase alpha-3 antibody
      • Ribosomal protein s6 kinase ii alpha 2 antibody
      • Ribosomal S6 kinase 2 antibody
      • Rps6ka3 antibody
      • RSK antibody
      • RSK-2 antibody
      • RSK2 antibody
      • S6 kinase 2 antibody
      • S6K alpha3 antibody
      • S6K-alpha-3 antibody
      see all

    Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] images



    • Predicted band size : 84 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (40 µg)
      Lane 4: (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue
    • Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + Hela cell lysate

      Predicted band size : 84 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    References for Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

    This product has been referenced in:
    • Takada I DGCR14 induces Il17a gene expression through the ROR?/BAZ1B/RSKS2 complex. Mol Cell Biol 35:344-55 (2015). WB, ChIP ; Mouse . Read more (PubMed: 25368387) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab32133.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"